Coding

Part:BBa_K4011010:Design

Designed by: Meilun Wu   Group: iGEM21_LINKS_China   (2021-10-21)


Ma-sfGFP-CBM3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 576
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 948
    Illegal AgeI site found at 3079
    Illegal AgeI site found at 3123
    Illegal AgeI site found at 3295
    Illegal AgeI site found at 3361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 424
    Illegal SapI.rc site found at 2320


Design Notes

1. The following mutations are added in Mα_Mut: A9D, A20T, Q32H, F48S, and G62R.

2. All codons were optimized for S. cerevisiae based on S. cerevisiae codon bias.

3. Linker EAEAFGS used between Ma and sfGFP, flexible linker GGGGS used between sfGFP and CBM3.


Source

Ma comes from S. cerevisiae, sfGFP is a highly characterized sequence used in synbio, CBM3 comes from Ruminiclostridium thermocellum.

References