Coding
Part:BBa_K4011010:Design
Designed by: Meilun Wu Group: iGEM21_LINKS_China (2021-10-21)
Ma-sfGFP-CBM3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 576
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 948
Illegal AgeI site found at 3079
Illegal AgeI site found at 3123
Illegal AgeI site found at 3295
Illegal AgeI site found at 3361 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 424
Illegal SapI.rc site found at 2320
Design Notes
1. The following mutations are added in Mα_Mut: A9D, A20T, Q32H, F48S, and G62R.
2. All codons were optimized for S. cerevisiae based on S. cerevisiae codon bias.
3. Linker EAEAFGS used between Ma and sfGFP, flexible linker GGGGS used between sfGFP and CBM3.
Source
Ma comes from S. cerevisiae, sfGFP is a highly characterized sequence used in synbio, CBM3 comes from Ruminiclostridium thermocellum.